ABSTRACT
Spirodela polyrrhiza is a floating plant widely used in biomass utilization and eutrophication phytoremediation. It becomes a common aquatic plant everywhere with the increasingly serious eutrophication. It has been reported that S. polyrrhiza has a good effect on the remediation of eutrophication water. In order to study the absorption and transportation of phosphorus in S. polyrrhiza, we extracted RNA from S. polyrrhiza and then reverse transcribed it into cDNA, which was used as a template to amplify a specific fragment. The full-length sequence of the open reading frame (ORF) was 1 620 bp, encoding 539 amino acids, named SpPHT1;1, and the accession number in GenBank was MN720003. Bioinformatical analysis showed that SpPHT1;1 had no intron. The protein it encoded was a stable, hydrophobic protein with 11 transmembrane domains. SpPHT1;1 structure was similar to that of major facilitator superfamily (MFS) superfamily members. The cluster analysis showed that SpPHT1;1 was closely related to ZMPHT2 in maize and SBPHT1-8 in sorghum. So, it might belong to plant PHT1 family. The expression of SpPHT1;1 in leaf was significantly more than that of root under normal phosphorus condition. Low phosphorus condition could promote gene expression, and the relative expression level of SpPHT1;1 arrived at the peak at 48 h both in root and leaf. High phosphorus condition could inhibit gene expression. These results indicated that SpPHT1;1 expression would be affected by external phosphorus concentration. The results of this study are helpful for further research on the function of phosphate transporter. It also can provide theoretical basis for further development and utilization of S. polyrrhiza.
Subject(s)
Araceae/genetics , Biodegradation, Environmental , Cloning, Molecular , DNA, Complementary , Phosphate Transport Proteins/geneticsABSTRACT
El ocumo (Xanthosoma sagittifollium (L.) Schott) es una Arácea cultivada en países tropicales debido al valor nutritivo de sus cormos. La principal limitante para su cultivo es la carencia de semilla de calidad, por esta razón se planteó evaluar la multiplicación de brotes de ocumo blanco en sistemas de inmersión temporal, y el enraizamiento ex vitro de los mismos, para lo cual se estudió el tiempo y la frecuencia de inmersión, y la densidad de explantes sobre la proliferación de los brotes. Asimismo, el efecto del ácido indolacético (AIA) y ácido indolbutírico (AIB) sobre el enraizamiento ex vitro de brotes. De acuerdo con los resultados obtenidos, la mayor eficiencia en la proliferación de brotes se obtuvo utilizando el sistema de inmersión temporal del tipo RITA®, con una frecuencia y tiempo de inmersión de 6 veces/día y 5 min, respectivamente, y una densidad de 9 explantes/RITA®. En el enraizamiento ex vitro se determinó que bajo las condiciones de cultivo empleadas no es necesario el uso de auxinas. Se concluye que es posible la multiplicación eficiente de ocumo blanco en sistemas de inmersión temporal, y realizar el enraizamiento ex vitro sin el uso de auxinas.
The white cocoyam (Xanthosoma sagittifollium (L.) Schott), is an Arácea cultivated in tropical countries, due to the nutritional value of its corms. The main limiting factor for cultivation is the lack of healthy seed, by this reason be outlined to evaluate the multiplication of shoots of white cocoyam in temporary immersion systems and the ex vitro rooting of the same. For that which, itself study, the time and frequency of immersion and the density of explants on the proliferation of the shoots. As well as, the effect of the indole acetic acid (IAA) and indole butyric acid (IBA) on ex vitro rooting the shoots was studied. According to the results obtained, the greater efficiency in the proliferation of shoots was obtained utilizing the temporary immersion system of the type RITA®, with a frequency and time of immersion of 6 times/day and 5 min, respectively and a density of 9 explantes/RITA®. In the ex vitro rooting was determined that under the conditions of employed cultivation is not necessary the use of auxins. It is concluded that is possible the efficient multiplication of white cocoyam in temporary immersion systems and to carry out the ex vitro rooting without the use of auxins.
Subject(s)
Araceae/growth & development , Araceae/adverse effects , Araceae/enzymology , Araceae/physiology , Araceae/genetics , Araceae/immunology , Araceae/microbiology , Araceae/parasitology , Araceae/chemistryABSTRACT
Although Agrobacterium-mediated transformation protocols for many economically important plant species have been well established, protocol for a number of flowering plants including Anthurium andraeanum remains challenging. In this study, we report success in generating transgenic Anthurium andraeanum cv Arizona using Agrobacterium GV3101 strain harboring a binary vector carrying gfp as a reporter gene. The possibility of facilitating the screening process for transgenic plants expressing functional proteins using gfp marker was explored. In order to realize high transformation efficiency, different explant sources including undifferentiated callus pieces and petioles were compared for their regeneration efficiency and susceptibility to Agrobacterium-mediated transformation. We also optimized the concentration of AS added to co-cultivation media. Genomic PCR revealed that 11 of the 22 resistant plantlets regenerated on selective medium were successfully transformed. Green fluorescence was observed using a fluorescence microscope in 7 of the 11 PCR-positive plants, indicating GFP was expressed stably in the transformed Anthurium andraeanum. The highest transformation efficiency obtained in this study was 1.71 percent (percentage of explants with transgenic shoots in total explants) when callus explants were used as starting material and 125 umol l-1 AS was added during the co-cultivation process.